Two distinct species of human growth hormone-variant mRNA in the human placenta predict the expression of novel growth hormone proteins.

Transcription of growth hormone mRNA as a molecular marker of egg quality in Siberian sturgeon (Acipenser baerii)

The Siberian sturgeon (Acipenser baerii) is an economically important species. Sturgeon stockassessment during fingerling production is considered to be one of the most difficult phases ofhatchery rearing. The present study investigated the expression of growth hormone (GH)mRNA in unfertilized eggs, fertilized eggs, eyed eggs (2 day before hatching), non-viable eggsand newly-hatched Siberian st...

CONSTRUCTION OF RECOMBINANT PLASMIDS FOR PERIPLASMIC EXPRESSION OF HUMAN GROWTH HORMONE IN ESCHERICHIA COLI UNDER T7 AND LAC PROMOTERS

In order to study the periplasmic expression of human growth hormone (hGH) in Escherichia coli, the related cDNA was inserted in two expression plasmids carrying pelB signal peptide, one with lac bacterial promoter and the other with a bacteriophage T7-based promoter. The recombinant plasmids were moved to TG1 and BL21 strains of E. coli, respectively. To induce the expression systems, IPTG and...

Comparison of Expression of Growth Hormone-Releasing Hormone and Its Receptor Splice Variant 1 in Different Stages of Endometriosis

P-66: Optimization of Human Luteinizing Hormone Expression in CHO Cells Culture by Stepwise Reduction in Serum Concentration

Background: Mammalian Cell lines are the main expression system for the production of recombinant therapeutic proteins. Optimization of cell culture condition is performed via alteration in different parameter. Cell culture media plays an important role in cell cycle because of compounds such as amino acids, vitamins, inorganic salts, glucose, and serum as a source of growth factors, trace elem...

P-98: The Effect of Primary pH Values of Medium Culture on Expression of Human Follicle Stimulating Hormone in Recombinant Chinese Hamster Ovary Cells.

Background One of the common gonadotropin hormones used for infertility treatment is recombinant Follicle stimulating hormone (rFSH). pH is an environmental factor that can affect production of recombinant proteins with no imposing cost. Culture pH is known as a parameter that significantly affects cell growth and protein production. In this study, tried to investigate the effect of different p...